Coding

Part:BBa_K5218009:Design

Designed by: Amy Fu, Juliette, Lichuan Chen, Xiaojuan Wang   Group: iGEM24_BGI-MammothEdu-South   (2024-09-03)


CrC3H


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1535
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1535
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1535
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nil


Source

The CrC3H gene loci is JAMLDZ010000192.1, transcript loci is M9H77_00625 (NCBI). Team BGI-MammothEdu-South 2024 extracted Catharanthus roseus total RNA, reverse transcribed into cDNA and cloned the CrC3H CDS with specific primer pairs.

References